Relationship between the expression of PD-1 on CD8+T cell and the clinical pathological parameters in non-small cell lung cancer

ZHOU Li; REN Fang; ZHANG Guannan; LV Xiying

Abstract：Objective To explore the expression characteristics of programmed death?1( PD?1) on CD8+T in the peripheral blood from patients with non?small cell lung cancer ( NSCLC) as well as the relationship between level of PD?1 and clinicopathologic variables. Methods Ninety NSCLC patients, including 50 squamous cell carcinoma and 40 adenocarcinoma, and thirty healthy con?trols were selected. CD8+T cells were obtained from the peripheral blood mononuclear cells. The positive expression ratio of the PD?1 on the CD8+T cell surfaces in the peripheral blood was detected using flow cytometry. We then analyzed the relationship of PD?1 expres?sion with age, gender, pathological classification, clinical stage, pathological degree and lymphatic metastasis. Results The positive expression ratio of PD?1 on CD8+T cell in the peripheral blood of the three groups were (18?32±5?20)%, (18?68±5?41)% and (7?44±2?59)%, and the data of NSCLC patients was higher than that of the healthy controls (F=58?344, P<0?001).The results were obviously related to the clinical stage and lymphatic metastasis ( P<0?05) , which positively correlated with the clinical stage of the NSCLC ( rs=0?875, P<0?001) . However, no difference was observed on age, gender, pathological classification and pathological degree ( P>0?05) . Conclusion The positive expression ratio of PD?1 on CD8+T cell in the peripheral blood of NSCLC patients was higher than that of the healthy controls, and the high expression ratio of PD?1 on CD8+T cell were significantly related to the clinical stages and lymphatic metastasis. The elevated level of PD?1 might be related with the immune escape of the NSCLC, thus playing a cru?cial role in the occurrence and development of NSCLC.