Effects of BRD4 inhibitor on proliferation, apoptosis and invasion of nasopharyngeal carcinoma cell line CNE-2

LIU Zhong; YANG Fei; CHENG Ji; TIAN Tianjie

Abstract：Objective To explore the effects of bromodomain?containing protein 4 ( BRD4) inhibitor( GSK525762A) on pro?liferation, apoptosis and invasion of nasopharyngeal carcinoma cell line CNE?2. Methods The nasopharyngeal carcinoma cell line CNE?2 was treated by GSK525762A ( 0, 0. 1, 1, 10, 100 μmol/L) for 24, 48, 72 and 96 h. The methyl thiazolyl tetrazolium salt ( MTT) was used to detect the proliferation inhibition rates at the above time points. Meanwhile, the apoptotic rates of CNE?2 cells at 48, 96 h after treatment with GSK525762A were measured with Annexin?FITC/PI double staining via flow cytometry. Transwell method was used to detect the invasion ability of CNE?2 cells at 48, 96 h after treatment with GSK525762A. The real?time quantitative PCR was performed to detect expressions of apoptosis related genes at 48, 96 h after treatment with GSK525762A. Results There was in?hibitory effect of GSK525762A on the proliferation of CNE?2 cells, and the inhibition rate was increased in a time?and concentration?dependent manner ( P<0. 05) . After the treatment of GSK525762A, the early, late and total apoptotic rates increased with the increase of concentrations, higher than those of 0 mol/L group ( P<0. 05);the number of transmembrane cells of other concentrations were low?er than that of 0 mol/L group ( P<0. 05);Compared with the 0μmol/L, there were lower level of mRNA in Bcl?2 but higher levels of mRNA in Bax and Bak with the range ( 0. 1?100 μmol/L) ( P<0. 05) . Conclusion BRD4 inhibitor GSK525762A presented toxicity on the proliferation of CNE?2 cell, induced apoptosis and restored the expressions of apoptosis related genes.