Expression of AEG-1 in oral squamous carcinoma and its correlation with prognosis

WANG Xiumei; GUO Yang; WEI Shenghua; CAO Xiaofang; XIE Xiaohua

Abstract：Objective To detect the expression of astrocyte elevated gene?1 ( AEG?1) in oral squamous cell carcinoma ( OS?CC) , and to investigate the relationship between expression of AEG?1 in OSCC and clinicopathological characteristics, prognosis. Methods The expression of AEG?1 was measured by Western blotting in OSCC cells( CAL27, SCC4, Tca8113, Tb, Ts) and HaCaT cell. At the same time, the expressions of AEG?1 of 87 patients with OSCC and 20 cases of normal oral mucosa were detected by immu?nohistochemistry. The relationship between the expression of AEG?1 in OSCC tissues and clinical pathological characteristics were ana?lyzed. The overall survival of OSCC patients was estimated using the Kaplan?Meier method. The factors affecting the prognosis of OSCC patients were analyzed by Cox proportional hazard regression model. Results AEG?1 was high expressed in OSCC cells ( CAL27, SCC4, Ts and Tb) . The high?level expression rates of AEG?1 was 51?7%( 45/87) and 10?0%( 2/20) , respectively in OSCC and nor?mal oral tissues, in which there existed difference statistically (P=0?001).The expression of AEG?1 was closely correlated with differ?entiation, clinical stage, T stage and lymph node metastasis. Univariate analysis showed AEG?1, clinical stage, T stage and lymph node metastases were prognostic factor of OSCC. Multivariate analysis showed AEG?1 was independent prognostic factors in OSCC( RR=1?948,95%CI:1?013?3?744,P=0?046) . Conclusion The overexpression of AEG?1 might play an important role in the occurrence and development of OSCC, and it can be used as a reference index of the malignant degree and poor prognosis of OSCC.