Effect and mechanism of scutellarein in suppressing the migration and proliferation of colorectal cancer HT-29 cells
ZHAO Xiaoyue
WANG Jiejun
YU Guanzhen
WANG Zhan
JIAO Xiaodong
LIU Ke
JIANG Longwei
Abstract：Objective To explore the effect of scutellarein on the migration and proliferation of colorectal cancer HT?29 cells as well as the underlying mechanism. Methods The MTT assay was used to investigate the proliferation of HT?29 cells after treatment with different doses of scutellarein(5, 10, 20, 40, 80 μg/ml). The effect of 80 μg/ml scutellarein on migration of HT?29 cells was measured by Transwell assay. Protein expression of signal transducer and activator of transcription(STAT3), nuclear factor(NF)?κB and p53 in HT?29 cells was assayed by Western blotting after treatment of 20, 40μg/ml scutellarein. Cells without treatment were as?signed as control group( 0 μg/ml scutellarein) . Results Compared with the control group, scutellarein presented inhibitory effect on the proliferation ability of T29 cells in a dose?dependent manner with significant difference( P<0?05) . There were decreased cell migra?tion ability and protein levels of STAT3 and NF?κB but increased level of p53 in scutellarein group versus control group(P<0?05). Conclusion Scutellarein significantly inhibited the migration and proliferation of HT?29 cells with the possible mechanism of increasing expression of p53 and decreasing levels of STAT3 and NF?κB in HT?29 cells.
Keywords：ScutellareinColon cancerProliferationMigration
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 208-211 )