Inhibition effect of miR-7 on proliferation and metastasis of Hep-2 laryngeal carcinoma cells

XU Yao; OUYANG Xuenong; CHEN Xi; ZHAO Zhongquan; QI Xiaoyan

Abstract：Objective To explore the effect of up?regulation of microRNA?7( miR?7) on proliferation and metastasis of Hep?2 laryngeal carcinoma cells and its possible mechanism. Methods The Hep?2 laryngeal carcinoma cells were divided into miR?7 group (Hep?2 cells transfected with miR?7 mimics), Scramble group(Hep?2 cells transfected with miR?Scramble) and Mock group(Hep?2 cells without transfection) . The quantitive real?time PCR( qPCR) was used to test the expression of miR?7 at 48 h after transfection in 3 groups. The CCK?8 assay was used to test the proliferation of Hep?2 cells in each group at 48 h after transfection. Cell metastasis was determined by transwell test at 48 h after transfection. The mRNA and protein levels of PI3K( p110) , AKT and pAKT were examined by qPCR and Western blotting. Results The expression of miR?7 was significantly up?regulated after transfection with miR?7 mimics. The proliferation and metastasis of Hep?2 laryngeal carcinoma cells was extremely decreased after transfection with miR?7. The results from qPCR and Western blotting showed that the mRNA and protein levels of PI3K( p110) , AKT and pAKT were decreased in miR?7 group compared with other two groups( P<0?05) . Conclusion Up?regulation of miR?7 can inhibit the proliferation and metastasis abil?ity of Hep?2 laryngeal carcinoma cells with possible mechanism of inhibiting PI3K/AKT signal pathway.