Influence of nm23-H1 gene transfection on expressions of MMP-9 and TIMP-1 in lung cancer cells
ZHENG Haixia
CUI Yongyan
SHEN Donglan
PENG An
HE Yanling
Abstract：Objective To investigate the influence of nm23?H1 gene transfection on the expressions of matrix metalloproterinase 9(MMP?9)and tissue inhibitor of metalloproteinase 1(TIMP?1) in lung cancer cells. Methods nm23?H1 gene was transfected into lung cancer cell line L9981 by gene transfection technology. Semi?RT?PCR was used to detect the different mRNA expressions of TIMP?1 and MMP?9 between transfected and non?transfected lung cancer cell lines. Flow cytometry was used to detect the different protein expressions of TIMP?1 and MMP?9 between transfected and non?transfected lung cancer cell lines. Results The mRNA and protein levels of TIMP?1 in transfected lung cancer cell lines were stronger than those in non?transfected lung cancer cell lines. The mRNA and protein levels of MMP?9 in transfected lung cancer cell lines were weaker than those in non?transfected lung cancer cell lines. Conclusion The nm23?H1 gene can up?regulate expressions of TIMP?1 and down?regulate expressions of MMP?9 in lung cancer cell line L9981.
Keywords：nm23-H1Lung cancer cell lineMatrix metalloproterinase 9Tissue inhibitor of metalloproteinase 1
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 104-107 )