Detection of EML4-ALK gene rearrangement in NSCLC by Ventana IHC and the challenges for diagnosis
WANG Xuan
YU Bo
MA Henghui
ZHOU Xiaojun
SHI Qunli
SONG Yong
WANG Jiandong
Abstract：Objective To analyze the occurrence of EML4?ALK gene rearrangement in non?small cell lung cancer ( NSCLC) detected by Ventana immunohistochemical staining (IHC), as to explore the challenges of Ventana IHC and provide a reference for other hospital to carry out the examination. Methods In this study, 659 cases of NSCLC were retrospectively analyzed and part of samples were checked by qRT?PCR. Results The occurrence rate of EML4?ALk in adenocarcinoma was 8?78% and in squamous cell carcinoma was 4?49%. Total occurrence in NSCLC was 8?48%. All cases with IHC staining (-) and (+) were confirmed negative mu?tation with qRT?PCR. Five cases with IHC staining (+++) were positive confirmed by qRT?PCR. One out of 5 cases with IHC staining (++) was confirmed positive by qRT?PCR. Conclusion EML4?ALK predominantly occurred in lung adenocarcinoma. There are some challenges in diagnosis of Ventana IHC. All cases with IHC staining (++) need to be verified by qRT?PCR or other methods.
Keywords：Non-small cell lung cancerVentana immunohistochemical stainingEML4-ALKGene rearrangement
Publication Date：2015-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 79-82 )