Expression and clinical value analysis of plasma miR-223, miR-93 and miR-218 in non-small lung cancer

CAI Jingqing; WANG Ning; ZHANG Min

Abstract：Objective To explore the levels of plasma miR?223, miR?93 and miR?218 in non?small lung cancer( NSCLC) and analysis the relationship between the 3 indicators and clinicopathological parameters of NSCLC. Methods Plasma samples from 85 NSCLC patients before treatment were collected as NSCLC group. The real?time quantitative RT?PCR( qRT?PCR) was used to detect the levels of miR?223, miR?93 and miR?218 and the clinicopathological parameters of NSCLC patients were collected. The clinicopatho?logical parameters of patients with different levels of miR?223, miR?93 and miR?218 were compared. The receiver operating characteris?tic curve( ROC) was employed to analysis the clinical value of plasma miR?223, miR?93 and miR?218 in the diagnosis of NSCLC. Meanwhile, the plasma samples from 90 healthy volunteers at the same time were selected as control( control group) . Results There were higher levels of miR?223 and miR?93 but lower miR?218 in NSCLC group compaired with control group with significant difference ( P<0?05) . The level of miR?223 was related with TNM stage and tumor size, and miR?93 was related with histological type and lymph node metastasis, and miR?218 was related with tumor size and degree of differentiation, showing with significant difference ( P all<0?05) . The plasma level of miR?223 was positively correlated with miR?93( r=0?411) , but miR?223 and miR?93 were negatively cor?related with miR?218(r=-0?361 and r=-0?451)with statistically significance(P<0?05). The AUC, sensitivity and specificity of plasma miR?223, miR?93 and miR?218 in the diagnosis of NSCLC were 0?926, 95?2% and 87?1%, 0?912, 88?4% and 92?5%, and 0?941, 92?7% and 84?4%, higher than 0?774, 75?1% and 66?2% of carcinoembryonic antigen( CEA) . The combined efficiency of miR?223, miR?93 and miR?218 in NSCLC was superior to that alone. Conclusion There are higher expression of miR?223 and miR?93 but lower expression of miR?218 in NSCLC. The plasma levels of the above indicators are related with clinical pathology parameters, showing a certain value in the diagnosis of NSCLC as assisted indicators in the diagnosis of NSCLC.