Influences of recombinant human endostatin on angiogenesis

YE Qing; QIN Shukui; YIN Xiaojin; LIU Yanhong; FENG Jundong; WU Qiong; QU Wenshu

Abstract：Objective To study the effects of recombinant human endostatin( endostar) on the angiogenesis?related biological behaviors such as chemotaxis, migration, adhesion, proliferation and tube formation of vascular endothelial cells. Methods With pri?mary cultured human umbilical vein endothelial cells( HUVEC) as a model, the fluorescence quantitative Boyden chamber analysis, wound healing assay, fluorescence quantitative adhesion assay, flow cytometry, tube formation assay and Matrigel plug test were em?ployed to evaluate the effects of endostar on the angiogenesis?related biological behavior of HUVECs in vitro and in vivo. Results En?dostar potently inhibited HUVEC migration in response of VEGF from 50 to 50000ng/ml, and its significant concentrations were 50ng/ml and 500ng/ml. Endostar inhibited HUVEC migration towards damage between 50ng/ml and 50000ng/ml in a dose?dependent man?ner. Compared with 0ng/ml, the endostar at the dose of 50, 500 and 5000ng/ml decreased adhesion rate and proliferation rate of HU?VEC cells as well as the number, area and length of HUVEC mesh tubular with statistically significant difference( P<0?05) . Matrigel plug test also revealed that Endostar inhibited Matrigel plug formation in SCID mice between 50ng/ml and 50000ng/ml. Conclusion At the cellular level, endostar inhibited the angiogenesis?related biological behaviors of HUVECs, including migration, adhesion, pro?liferation and tube formation. In vivo, endostar inhibited the angiogenesis in SCID mice.