Effects of Nrf2 gene silencing by RNA interference on biological behaviors of esophageal carcinoma cells
ZHANG Jun
KONG Deyou
ZHANG Jian
KONG Jie
ZHANG Andu
Abstract：Objective To investigate the effect of down?regulation of nuclear factor erythroid 2?related factor( Nrf2) by RNAi on biological behaviors in esophageal cancer cells. Methods Eca?109 cell line which was down?regulated Nrf2 by RNAi( Si?Nrf2) and negative control cell( Si?control) were established. Si?Nrf2 cells and Si?control cells were transfected by Lipofectamine 2000. Cell prolif?eration, apoptosis, cell metastasis and relative protein expression were analyzed by MTT assay, colony formation assay, flow cytometry, transwell and western blotting method after transfection. Results Western blotting showed that the expression of Nrf2 in the Si?Nrf2 cells was 0?209±0?013, which was significantly lower than that in the Si?control cells(0?852±0?077, P<0?05). The result of biologi?cal function shown that Eca?109 cell down?regulated Nrf2 had a lower survival fraction, higher cell apoptosis, and significant decrease in migration and invasion, higher radiosensitivity and lower expression of HO?1, Bcl?2, MMP?9 protein compared with those of Si?con?trol cells( P<0?05) . Conclusion Nrf2 may involve biological process of cell proliferation, apoptosis, invasion and metastasis and ra?diation sensitivity in esophageal cancer by regulating the expression of HO?1, Bcl?2 and MMP?9 protein.
Keywords：Nrf2Esophageal squamous cell carcinomaCell proliferationCell apoptosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 1057-1061 )