Expression and function of miR-148b in lung adenocarcinoma

FANG Xisheng; LIU Guolong; LIU Xia; LV Lin

Abstract：Objective To investigate the expression and clinical significance of miRNA-148b ( miR-148b) in lung adenocar-cinoma ( LAC) tissues and cell lines. Methods Realtime-PCR was performed to detect the expression of miR-148b between 13 cases of LAC tissues and matched adjacent normal tissues. The expression of miR-148b was also detected in normal lung epithelial HBE cell line, and LAC cell lines H1437, H1975, A549 and PC14/B. The cell line with lowest expression was respectively transfected with miR-148b mimics and miR-148b control. Then, the effect of miR-148b on proliferation, colony formation and invasion ability were ana-lyzed by MTT, low-density cell colony formation and transwell experiments. Results The average relative expression of miR-148b in LAC tissues and adjacent normal tissues were 0. 61±0. 42 and 0. 91±0. 32 (P<0. 05). The expression levels of miR-148b in the H1437, H1975, A549, PC14/B cell lines were 0. 42±0. 08,0. 38±0. 02,0. 29±0. 03 and 0. 21±0. 04, lower than that in HBE cell line ( the expression level was set as 1. 00) with significance ( P<0. 05) . PC14/B cell line was selected for the following experiments. MTT analysis showed that the absorbance value of miR-148b mimics group was much lower than that of miR-148b control group from the 3th day of the test ( P<0. 05) . As for the low-density cell colony formation analysis, the relative colony formation number of PC14/B cells transfected with miR-148b mimics was 47±8, which was decreased significantly compared with miR-148b control group (set as 100) with significance ( P<0. 05) . Transwell test showed that there was significant difference in the relative numbers of cells migrated through the basement membrane between miR-148b mimics group (82±22) and miR-148b control group (200±34) with significance ( P<0. 05) . Conclusion The expression of miR-148b is down-regulated in both LAC tissue and cell lines. It can suppress the cell proliferation,colony formation and invasion ability of PC14/B cell in vitro.