An investigative and evaluative experimental scheme of antiangiogenesis of hepatocellular carcinoma
YE Qing
YIN Xiaojin
LIU Yanhong
FENG Jundong
WU Qiong
QU Wenshu
QIN Shukui
Abstract：Objective To develop a set of methods for studying and evaluating the angiogenesis in hepatocellular carcinoma ( HCC) . Methods Human umbilical vein endothelial cells ( HUVEC) was employed as the model and normal hepatocyte line L02 was control. A series of classical in vivo and in vitro experiment were modified and then applied during the research to actually and precisely describe the phenomenon of HCC induced angiogenesis, such as Boyden chamber invasion assay, fluorescence quantitative adherence assay, scratch test,flow cytometry, quantitative analysis using Image-Pro Plus and animal experiment of matrigel-plug. Results HCC conditioned media ( HCM) was more potent than normal hepatocyte conditioned media ( NCM) in inducing migration of HUVEC with significant difference( P<0.05) . HepG2 adhered to HUVEC easier than L02 did( P<0.05) . Co-culture of HepG2 and HUVEC promoted proliferation of HUVEC significantly in contrast to L02( P<0.05) . Co-culture system of HCM, HepG2 and HUVEC facilitated HUVEC to form net-like structure, while that of NCM, L02 and HUVEC did not. In vivo experiment also revealed that HCC was more potent than normal hepatocyte on angiogenesis. Conclusion The results indicated that this set of modified classical in vivo and in vitro experi-ments can be applied for the study and evaluation of antiangiogenic agents in HCC-induced angiogenesis.
Keywords：Hepatocellular carcinomaAngiogenesisBiological behaviorsVascular endothelial cell
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 783-789 )