Effect of NVP-BEZ235 on proliferation, apoptosis, invasion and metastasis of HepG2 cells

WEI Juan; QI Jiping; ZHENG Qin

Abstract：Objective To explore the effect of NVP-BEZ235 on the proliferation, apoptosis, invasion and metastasis of HepG2 Cells. Methods The HepG2 cells were treated with different concentrations of NVP-BEZ235(0, 0?01, 0?1, 1?0μmol/L). MTT was used to study the proliferation inhibition rates at 24, 48, 72 and 96h treated with different concentrations of NVP-BEZ235. Transwell assay was used to check the invasion and metastasis of HepG2 cells with NVP-BEZ235. The cycle distribution at 48h after treatment with NVP-BEZ235 was detected by flow cytometry. The RT-PCR and Western blotting were used to measure the change of MMP-2 expression. Results NVP-BEZ235 can increase the proliferation inhibition rates of HepG2 cell in a dose-and time-dependent manner. In addition to the late apoptosis rate of 0?01μmol/L at 24h, the early and late apoptosis rates of the remaining concentrations were higher than those of 0μmo/L at 24h and 48h(P<0?05). The proportion of cells in G0/G1 phase of 0?01, 0?1 and 1?0μmol/L NVP-BEZ235 were higher than those of 0μmo/L, while the transmembrane cell number, the proportion of cells in G2/M and S phase, and the protein and mRNA levels of MMP-2 of 0?01, 0?1 and 1?0μmol/L NVP-BEZ235 were lower than those of 0μmo/L at 48h( P<0?05) . Conclusion NVP-BEZ235 effectively inhibits the abilities of proliferation, invasion and metastasis abilities of HepG2 cells, promotes the apoptosis, arrests cells at G0/G1 phase and decreases the expression of MMP-2.