Influences of Schisandrin B on proliferation, apoptosis and Wnt/β-catenin signaling pathway of Skov3 hu-man ovarian cancer cells

ZENG Wenqiong; XU Qing; XU Yinyan

Abstract：Objective To explore the influences of Schisandrin B ( Sch B) on proliferation, apoptosis and Wnt/β-catenin signaling pathway of SKOV3 human ovarian cancer cells. Methods The SKOV3 cells were treated with different concentrations of Sch B ( 0, 1?0, 10?0, 20?0, 50?0μmol/L) . The MTT was used to measure the proliferation inhibition rates at 24, 48, 72 and 96h treated with different concentrations of Sch B. The Annexin-FITC/PI double staining was employed to detect cell apoptosis at 48h after treat-ment with different concentrations of Sch B. The cycle distribution at 48h after treatment with Sch B was detected by flow cytometry. The Western blotting was used to measure the nuclearβ-catenin protein levels in Wnt/β-catenin signaling pathway as well as its downstream target C-myc and cyclin D1. The cytoplasm/nucleus activities of glycogen synthase kinase 3β ( GSK-3β) at 48h after treatment with different concentrations of Sch B were measured by activity assay kit. Results The Sch B can increase the proliferation inhibition rates in a dose-and time-dependent manner, and elevate the early and late apoptosis and cytoplasm/nucleus activities of GSK-3βat 48h after treatment. In addition to 1?0μmol/L, the proportion of cells in G0/G1 phase of the remaining concentrations were higher than those of 0μmo/L ( P<0?05) , and the proportion of cells in S phase and G2/M phase andβ-catenin, C-myc and Cyclin D1 protein levels were lower than those of 0μmo/L ( P<0?05) . Conclusion Sch B can inhibit the proliferation, promote apoptosis and cell cycle arrest and inhibit the activation of Wnt/β-catenin pathway.