Expression of miR-122 in hepatocarcinoma cell strains and its relationship with PTEN
ZHANG Ying
JIA Shaochang
XIANG Fang
LU Lei
WANG Xuan
Abstract：Objective To investigate and analyze the expression of miR-21 in hepatocarcinoma cell strains and the relation-ship with PTEN. Methods The quantitative reverse transcription-PCR were used to detect the expression of miR-122 and PTEN in hepatocarcinoma cell strains, PTEN and p-Akt expression was detected in tumor-bearing nude mice treated with miR-21 inhibitor. Re-sults The relative expression of miR-21 was 2?97±0?79,137?66±23?60 and 11?21±1?14 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 1?29 ± 0?33, 36?90 ± 11?84 and 6?22 ± 0?85 respectively when miR-21 inhibitor was transfected. The relative expression of PTEN was 2?88 ± 0?93, 0?19 ± 0?07 and 0?62 ± 0?06 in WRL-68, MHCC97-H and SMMC-7721strains respectively, while that was 6?11±1?07, 2?65±0?66 and 4?32±0?84 respectively when miR-21 inhibitor was transfected. The expression of PTEN was positive and p-Akt was down regulated when tumor-bearing nude mice was treated with miR-21 inhibitor. Conclusion MiR-21 is an upstream regulation factor of PTEN, it can affect PTEN expression.
Keywords：HepatocarcinomamiR-21PTENExpression
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 490-493 )