The regulatory mechanism of RhoA on VEGF expression in esophageal squamous cell carcinoma cell line Eca-109
MA Ji
ZHAO Qingli
ZHONG Cuiping
LIU Wenchao
XUE Yan
Abstract：Objective To explore the effect and mechanism of RhoA on vascular endothelial growth factor( VEGF) expression and regulation in esophageal carcinoma cell line Eca-109. Methods Eca-109 cells were transfected by RhoA up-regulating plasmid (V14RhoA) or down-regulating plasmid(shRhoA) and VEGF expression level was examined by Western blotting and ELISA assay. The effect of RhoA on p53 expression was examined by Western blotting and real-time PCR. Results Western blotting and ELISA as-says showed that in Eca-109 cells VEGF protein expression was increased or decreased with up-regulating or down-regulating RhoA. Western blotting and real-time PCR assays showed that increased RhoA could inhibit p53 expression and then promote VEGF expres-sion. Conclusion In Eca-109 cells, RhoA can regulate VEGF expression, and RhoA promotes VEGF expression through inhibiting p53 expression.
Keywords：Ras homolog gene family/member A(RhoA)Esophageal carcinomaVascular endothelial growth factor ( VEGF)p53
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 486-489 )