Laboratory diagnosis of myeloid/nature killer cell precursor acute leukemia:one case report
WANG Jianning
BAO Hongyu
MENG Qingqi
SONG Min
ZHANG Liubo
HOU Yanqiu
JIANG Suyu
FU Xingcai
Abstract：Objective To analyze the diagnostic process of a rare case diagnosed of myeloid/nature killer cell precursor acute leukemia( M/NKPAL) and to improve the recognition of M/NKPAL. Methods Cell morphology was analyzed by marrow smear and re-lated cell chemical staining. Immunophenotyping of blast cells was performed by flow cytometry. Cytogenetics technique was used for karyotype analysis. PCR was applied for detection of T-cell recepter gene rearrangement or fusion gene associated with leukemia. The related articles were reviewed. Results With regard to morphology, most of leukemic cells demonstrated that 90?4% blasts in the bone marrow were generally L2-shaped with negative reactivity of myeloperoxidase staining and Auer?s rods were occasionally found. Expres-sion of CD34, HLA-DR,CD33,CD7,CD56,CD38 and cyCD3( dim) ,but no other markers including cyMPO,CD3 and CD4 were ob-served. Cytogenetics examination revealed abnormal karyotype. Clonal T-cell recepter gene rearrangement and fusion gene were not de-tected. Conclusion M/NKPAL is considered extremely rare and diagnosis is difficult depending merely on the morphology. It is im-portant to be distinguished from T-cell acute lymphoblastic leukemia with expression of myeloid-antigen, acute myeloid leukemia with minimal differentiation, mixed-phenotype acute leukemia with T/myeloid lineage and blastic NK cell leukemia/lymphoma.
Keywords：Acute leukemiaMyeloidNatural killer cellImmunophenotypingT-cell receptor gene
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 258-262 )