Expression of miR-455-3p and its relationship with MVD and VEGF in gastric carcinoma

SONG Jianqing; CAO wei; LYU Haoyue

Abstract：Objective To investigate the level of miR-455-3p in gastric carcinoma and its relationship with microvascular density ( MVD) and vascular endothelial growth factor ( VEGF) . Methods The quantitative real-time PCR was used to examine the level of miR-455-3p in 80 gastric carcinoma tissues ( gastric carcinoma group) . The vascular endothelial growth factor ( VEGF) expres-sion and microvascular density ( MVD ) were measured by immunohistochemical method in 80 gastric carcinoma tissues. The correlations between level of miR-455-3p and MVD, VEGF expression were analyzed. Thirty-seven superficial gastritis and 12 normal gastric mucosa tissues were chosen as control ( control group) . Results The level of miR-455-3p in gastric carcinoma group was 1.16 ±0.59, lower than 2.61±0.88 in control group with significant difference (P<0.05). The gastric carcinoma group.s positive expression rates of VEGF and MVD were 71.3% (57/80) and 54.9±7.3, higher than 28.6% (14/49) and 27.5±6.1 in control group with sig-nificant difference ( P<0.05) . The miR-455-3p was negatively correlated with VEGF expression ( r=-0.783, P<0.05) and MVD ( r=-0.824, P<0.05) in gastric carcinoma. Conclusion There was a decreased level of miR-455-3p in gastric carcinoma. The miR-455-3p was negatively correlated with VEGF expression and MVD in gastric carcinoma, which may play an important role in the regula-tion of blood vessel growth.