Influence of CDCA5-siRNA on proliferation and apoptosis of human hepatocarcinoma cell line HepG2

CHEN Shuzhen; NIE Yuqiang; DU Yanlei; XU Yan; SHEN Guijia

Abstract：Objective To investigate influence of CDCA5 small interfering RNA( CDCA5-siRNA) on proliferation and apop-tosis of human hepatocarcinoma cell line HepG2. Methods The HepG2 cells were divided into siRNA groups ( A, B and C groups) , universal scrambled negative control siRNA group ( NC group) and Blank group. Three CDCA5-siRNAs with different sequences were transfected into HepG2 cells respectively. Western blotting method was used to measure expressions of CDCA5 protein levels at 72h af-ter transfection. The cell proliferation was assessed by CCK-8 at 24, 48, 72, 96 and 120h after transfection. The Annexin V-FITC/PI double-labeled flow cytometry was employed to measure the apoptosis at 48h after transfection. Results The expressions of CDCA5 protein significantly decreased in the CDCA5-siRNA groups ( A,B and C groups) compared with NC group and Blank group( P<0.05) . Group B had the lowest expression rate and highest inhibition rate( 89.3%) . The relative proliferation rate of group B was significantly lower than those of NC group and Blank group since 48 hours after transfection( P<0.05) , and the lowest proliferation rate was ( 66.58 ±2.58)% at 72h in group B. The early and late apoptosis rates were (17.43±2.31) % and (22.37±2.21) % in B group, higher than those of other two groups( P<0.05) . Conclusion siRNA down-regulating the expression of CDCA5 gene in human hepatocarcino-ma cell line HepG2 can inhibit cell proliferation and increase cell apoptosis.