Effect of LASP1 on proliferation and apoptosis of undifferentiated thyroid cancer cells by regulating parathyroid hormone-related proteins
BAI Tubuxin
SHEN Guangtai
HE Heping
ZHANG Zhipeng
ZHANG Yuhai
TIAN Ruibo
YU Xiaoqiang
SUN Tianyang
HOU Tian
DING Xiaoli
LI Qingping
LAN Lili
Abstract：Objective To investigate the effect of LIM and SH3 protein 1(LASP1)on the proliferation and apoptosis of undifferentiated thyroid cancer cells by regulating parathyroid hormone-associated protein(PTHRP).Methods Immunohistochemical staining and Western blotting were used to analyze the expression level of LASP1 in undifferentiated thyroid cancer tissues and adjacent tissues.The undifferentiated thyroid carcinoma Hth74 cells were divided into the si NC group,the si LASP1 group and the si PTHRP group.The proliferation activity of Hth74 cells was analyzed by CCK-8 assay and cell colony formation assay,and the apoptosis rate of Hth74 cells was analyzed by in situ end-labeling method.The expression levels of LASP1 and PTHRP in Hth74 cells were detected by Western blotting.Results The expression level of LASP1 in cancer tissues was higher than that in adjacent tissues(P<0.01).Compared with the si NC group,the proliferation ability of Hth74 cells in the si LASP1 group and the si PTHRP group was decreased(P<0.05).Compared with the si NC group,the apoptosis rate of Hth74 cells in the si LASP1 group and the si PTHRP group was increased(P<0.05).Compared with the si NC group,the expressions of LASP1 and PTHRP in the si LASP1 group were both decreased,and the expression of PTHRP in the si PTHRP group was decreased(P<0.05).Conclusion In undifferentiated thyroid cancer tissues,the expression of LASP1 is increased,and the expression of PTHRP is decreased after inhibiting LASP1.Reducing the expression of LASP1 or PTHRP can inhibit the proliferation of undifferentiated thyroid cancer cells and promote its apoptosis.
Keywords：undifferentiated thyroid carcinomaLIM and SH3 proteins 1parathyroid hormone-related proteinapoptosisHth74 cellscell proliferation
Publication Date：2025-09-13
Online Publishing Date：2025-09-23(First online date of this platform, not the publication date of the document)
Pages：5( 70-74 )