Expression Levels of miR-27a and IL-27 in Peripheral Blood of Hashimoto Thyroiditis Patients and Their Clinical Significance

ZHANG Zhiping; FANG Yan; LIU Jiayun

doi:10.3969/j.issn.1002-3429.2025.11.005

Abstract：Objective To investigate the expression and clinical significance of microrNA-27a(miR-27a)and interleukin-27(IL-27)in peripheral blood of patients with Hashimoto's thyroiditis(HT).Methods A total of 120 patients with HT admitted from January 2022 to February 2024 were selected as the HT group,and 100 healthy people who underwent physical examination during the same period were selected as the control group.The general data and thyroid function parameters of the two groups were collected,and miR-27a and IL-27 in peripheral blood were detected and compared between the two groups.Pearson correlation analysis was used to analyze the relationship between miR-27a,IL-27 and thyroid function parameters.Receiver operating characteristic(ROC)curve was used to analyze the diagnostic value of miR-27a and IL-27 levels in HT.Results The proportion of family history of thyroid disease,thyroid stimulating hormone(TSH),thyroglobulin antibody(TgAb),thyroid peroxidase antibody(TPOAb),miR-27a and IL-27 in the HT group were significantly higher than those in the control group.Free triiodothyronine(FT3)and free thyroxine(FT4)were lower than those in the control group(P<0.05 or P<0.01).The levels of miR-27a and IL-27 were positively correlated with TSH,TgAb and TPOAb in patients with HT(P<0.05 or P<0.01),but were not significantly correlated with FT3 and FT4 in patients with HT(P>0.05).Multivariate logistic regression analysis showed that family history of thyroid disease,high level of TgAb,high level of TPOAb,high level of miR-27a and high level of IL-27 were risk factors for HT(P<0.05 or P<0.01).ROC curve analysis showed that the area under the ROC curve of combined detection of miR-27a and IL-27 was significantly higher than that of single diagnosis(P<0.01).Conclusions The levels of miR-27a and IL-27 in peripheral blood of patients with HT are highly expressed,and are related to thyroid function and antibodies.The elevated levels of miR-27a and IL-27 increase the risk of HT,and have certain value in the early diagnosis of HT.