Study on Synergism and Attenuation Effect of Vinegar-baked Bupleurum in Mice with Depression
SU Dan
LI Qianmin
LUO Jian
SONG Yonggui
Abstract：Objective To investigate the synergism and attenuation effect of vinegar-baked Bupleurum in mice with depression.Methods Seventy male C57 mice were randomly divided into control group,model group,Paroxetine group(10 mg/kg),low-dose raw Bupleurum group(1.5 g/kg),high-dose raw Bupleurum group(12 g/kg),low-dose vinegar-baked Bupleurum group(1.5 g/kg)and high-dose vinegar-baked Bupleurum group(12 g/kg),with 10 mice in each group.Except the control group,mice in other groups were treated with chronic unpredictable mild stress(CUMS)method to build depression model,and then given corresponding drug treatment for 15 d,and the control group and the model group were re-placed with an equal volume of normal saline.Depression-like behavior of mice was evaluated by sugar water consumption test,open field test and forced swimming test,and liver function of mice in each group was detected.Pathological changes of liver tissue were observed by HE staining,and lipid peroxide content in liver tissue was detected by enzyme-linked immunosor-bent assay(ELISA).Results Compared with the control group,the sugar water preference rate and the time of entering the center area of the open field were decreased in the model group,while the motionless time of forced swimming was prolonged,and the contents of alanine aminotransferase(ALT),aspartate aminotransferase(AST),total bile acid(TBA),malondialde-hyde,4-hydroxynonenal and leukotriene B4 in serum were increased in the model group(P＜0.05).Compared with the model group,the sugar water preference rate of low-dose raw Bupleurum group,low-dose and high-dose vinegar-baked Bupleurum groups and Paroxetine group was significantly increased,and the time of entering the central area of the open field in low-dose and high-dose raw Bupleurum groups,low-dose and high-dose vinegar-baked Bupleurum groups and Paroxetine group was in-creased;the motionless time of forced swimming was shortened.ALT,AST,TBA,4-hydroxynonenal and leukotriene B4 were significantly decreased,and malondialdehyde was decreased in low-dose and high-dose vinegar-baked Bupleurum groups and Par-oxetine group(P＜0.05),and the low-dose vinegar-baked Bupleurum group had the optimal efficacy.There was improvement in the pathological changes of liver tissues in raw Bupleurum groups and vinegar-baked Bupleurum groups.Conclusion The syn-ergism and attenuation effect of vinegar-baked Bupleurum was better than that of raw Bupleurum on mice with depression.
Keywords：DepressionBupleurumVinegar-bakedAlanine aminotransferaseAspartate aminotransferaseMalon-dialdehydeLeukotriene B4Mice
Publication Date：2024-11-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 85-89,100 )