Application of Detection of Pathogenic Microorganism in BALF by mNGS in Refractorily Treated Pneumonia
WANG Na
ZHANG Fang
MENG Jun
ZHAO Zhidong
XU Yunduo
Abstract：Objective To investigate the application effect of detection of pathogenic microorganism in bronchoalveo-lar lavage fluid(BALF)by metagenomic next-generation sequencing technology(mNGS)in patients with refractorily treated pneumonia(RTP).Methods The data of 60 RTP patients who underwent bronchoscopic mNGS of BALF(mNGS group)and 60 RTP patients who underwent traditional culture(traditional group)from January 2023 to January 2024 were retrospec-tively analyzed.The positive rate,pathogen type,infection type and treatment were compared between mNGS group and tradi-tional group.Results The positive detection rate of pathogenic bacteria in mNGS group was 95.00%(57/60),which was higher than that of traditional group(71.67%,43/60)(P＜0.05).In all,84 strains and57 strains were detected in the mNGS group and the traditional group,respectively.The positive detection rates of gram-negative bacteria and gram-positive bacteria in mNGS group were lower than those in traditional group,while the positive detection rates of fungi,viruses and spe-cial pathogens in MNGS group were higher than those in traditional group(P＜0.05,P＜0.01).The detection rate of single infection in traditional group was higher than that in mNGS group(P＜0.01),and bacterial infection was the main one.The detection rate of mixed infection in mNGS group was higher than that in traditional group(P＜0.01),and virus-bacterial in-fection and virus-bacteria-fungal infection were the main ones.The duration of antibiotic use,frequency of antibiotic adjust-ment,length of hospital stay and hospitalization expenses in mNGS group were shorter or lower than those in traditional group,and the total effective rate was higher than that in traditional group(P＜0.05,P＜0.01).Conclusion Detection of patho-genic microorganism in BALF by mNGS has a good application effect in the etiological diagnosis of RTP patients,which can help clinicians identify pathogens early and then implement precise treatment,improve the total effective rate of treatment and reduce complications.
Keywords：Refractorily treated pneumoniaInfectionAlveolar lavage fluidMetagenomic next-generation sequen-cing technologyPathogenic bacteriaDetection methodTherapeutic effect
Publication Date：2024-09-15
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 43-47 )