Relationship between C3,C4,Immunoglobulin and EBV-DNA Load in Children with Infectious Mononucleosis and Efficacy of Combined Diagnosis
HE Jing
XIAO Changshui
SHU Li
Abstract：Objective To investigate the relationship between complement 3(C3),complement 4(C4),immunoglobu-lin(Ig)A,IgG,IgM and Epstein-Barr virus(EBV)-DNA load in children with infectious mononucleosis(IM)and the combined diagnostic efficacy.Methods A total of 76 children with IM treated from June 2020 to December 2023 were selected as the research group,and 76 healthy children were selected as the control group at a ratio of 1∶1.The expression of serum C3,C4,IgA,IgG and IgM were compared among those with different gender,age,course of disease,number of systemic damage and EBV-DNA load between the two groups.The correlation between the course of disease,number of systemic damage,EBV-DNA load and serum C3,C4,IgA,IgG and IgM in children with IM was analyzed by Spearman analysis.Receiver operating charac-teristic(ROC)curve was drawn to analyze the efficacy of the above indicators alone and in combination in the diagnosis of IM.Results Compared with the control group,the levels of serum C3,C4,IgA,IgG and IgM in the research group were higher(P<0.01).The expressions of serum C3,C4,IgA,IgG and IgM in the research group were as follows:acute stage>recovery stage,1 system injury<more than 1 system injury,EBV-DNA high load>medium load>low load,and the difference was statis-tically significant(P<0.01).The course of disease of IM children was negatively correlated with serum C3,C4,IgA,IgG and IgM,while the number of systemic damage and EBV-DNA load were positively correlated with serum C3,C4,IgA,IgG and IgM(P<0.01).The area under the ROC curve(AUC)of serum IgA,IgG and IgM combined with C3 and/or C4 or the combination of the above five indicators in the diagnosis of IM was better than that of single indicator,and IgA+IgG+IgM+C4+C3 was the opti-mal combination,with the sensitivity of 0.908 and the specificity of 0.842.Conclusion The abnormal expression of C3,C4,IgA,IgG and IgM in children with IM is closely related to the course of disease,the number of systemic damage and EBV-DNA load.Serum C3,C4,IgA,IgG,and IgM in combination has a higher value in the diagnosis of IM.
Keywords：Infectious mononucleosisChildrenComplement 3Complement 4ImmunoglobulinCourse of diseaseViral loadCorrelationDiagnostic efficacy
Publication Date：2024-07-28
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：7( 76-82 )