Study on Mechanism of Propofol Inhibiting Proliferation,Migration and Invasion of Prostate Cancer Cells
ZHAO Cuidang
SHI Jiong
ZHAO Honglei
ZHAO Guangping
HE Ping
Abstract：Objective To investigate the effects of Propofol on the proliferation,migration and invasion of prostate cancer(PCa)cells and its regulation on lncrNA-related lung adenocarcinoma transcript 1(MALAT1)/miR-185-5p.Meth-ods Human PCa DU145 cells were treated with different concentrations of Propofol or 80 μmol/L Propofol for 24,48 and 72 h.CCK-8 method,EdU staining,scratch test and Transwell test were used to detect cell viability,proliferation,migration and invasion ability,respectively.The expressions of lncRNA MALAT1 and miR-185-5p were detected by qRT-PCR,and the expressions of Ki67,E-cadherin,N-cadherin and Vimentin were detected by immunoblotting assay.The effects of MALAT1 and miR-185-5p on cell proliferation,migration,and invasion were detected after transfection of oe-MALAT1 and miR-185-5p mimic with Propofol.The targeting relationship between MALAT1 and miR-185-5p was analyzed by bioinformatics and dual lu-ciferase reporting experiments.Results The viability of DU145 cells was significantly decreased after treatment with≥40 μmol/L Propofol,and the cytotoxicity was time-dependent after treatment with 80 μmol/L Propofol(P＜0.01).Com-pared with the control group,Propofol significantly inhibited cell proliferation,migration and invasion,significantly down-regulated the expressions of Ki67,N-cadherin and Vimentin,and up-regulated the expression of E-cadherin(P＜0.01).The expression of MALAT1 was significantly down-regulated and the expression of miR-185-5p was up-regulated(P＜0.05,P＜0.01).Overexpression of MALAT1 significantly reversed the effect of Propofol on DU145 cells.Dual luciferase assay con-firmed that MALAT1 targeted binding and negatively regulated the expression of miR-185-5p. Compared with the Propofol + oe-MALAT1 +miR-NC group, the rate of EdU positive cells and cell migration rate and the number of invasive cells of the Propofol +oe-MALAT1 +miR-185-5p group were significantly decreased, and the expressions of Ki67, N-cadherin and Vim-entin were significantly down-regulated. E-cadherin expression was significantly up-regulated (P ＜ 0. 01). Conclusion Propofol can significantly inhibit the proliferation, migration and invasion of PCa DU145 cells, and its mechanism is related to lncRNA MALAT1/miR-185-5p axis.
Keywords：Prostatic neoplasmsPropofolMALAT1miR-185-5pCell proliferationCell movementCell inva-sionMechanism
Publication Date：2023-07-22
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：9( 59-67 )