Relationship between NF-κB mRNA,miR-125a-5p and Clinicopathological Features and Prognosis in Patients with Thyroid Cancer

ZHU Meng; SONG Jia; GUO Jianmei

doi:10.3969/j.issn.1002-3429.2023.07.013

Abstract：Objective To investigate the relationship between nuclear transcription factor(NF-κB)mRNA,miR-125a-5p and clinicopathological features and prognosis in patients with thyroid cancer.Methods From May 2017 to April 2020,240 patients with thyroid cancer undergoing surgery were selected as the observation group,and 60 healthy physical ex-aminees during the same period were selected as the control group.The levels of NF-κB mRNA and miR-125a-5p were com-pared between the two groups,and the relationship between NF-κB mRNA,miR-125a-5p levels and clinicopathological char-acteristics of thyroid cancer patients was analyzed,and clinical data of patients with and without recurrence were compared.The relationship between NF-κB mRNA and miR-125a-5p levels and postoperative recurrence of thyroid cancer and the corre-lation between NF-κB mRNA and miR-125a-5p levels were compared.The predictive value of NF-κB mRNA and miR-125a-5p detection in postoperative recurrence of thyroid cancer was evaluated by receiver operating characteristic(ROC)curve.Results The expression level of NF-κB mRNA in observation group was higher than that in control group,and the level of miR-125a-5p was lower than that in control group(P＜0.05).There were significant differences in NF-κB mRNA and miR-125a-5p levels in tumor size,clinical stage,vascular space invasion and lymph node metastasis in patients with thy-roid cancer(P＜0.01).The tumor size＞5 cm,clinical stage Ⅲ-Ⅳ,proportion of vascular space infiltration and lymph node metastasis and NF-κB mRNA level at 1 week after surgery for thyroid cancer in patients with recurrence were higher than those in patients without recurrence;the level of miR-125a-5p and the absolute difference of NF-κB mRNA and miR-125a-5p before and after surgery were lower than those without recurrence(P＜0.05,P＜0.01).The absolute difference of NF-κB mRNA and miR-125a-5p before and after surgery was an influential factor for postoperative recurrence of thyroid cancer pa-tients(P＜0.01).The area under the ROC curve of the absolute difference of NF-κB mRNA and miR-125a-5p before and af-ter surgery in combination for prediction was 0.927,which was higher than that predicted by each index alone(0.831,0.775 respectively)(P＜0.05).Correlation analysis showed that NF-κB mRNA was negatively correlated with miR-125a-5p in thy-roid cancer patients(r =-0.765,P＜0.01).Conclusion NF-κB mRNA level is up-regulated and miR-125a-5p expres-sion level is down-regulated in thyroid cancer patients.Clinical detection of the absolute difference before and after surgery can predict the recurrence of thyroid cancer,which is conductive to early postoperative adjuvant therapy.