Effects of Fisetin on Osteoporosis in Rats Undergoing Ovariectomy
YANG Xin
LI Yuanli
JIANG Ping
WANG Ye
JING Xueming
Abstract：Objective To investigate the effects of Fisetin on osteoporosis in rats undergoing ovariectomy.Methods A total of 50 female SD rats were treated with bilateral ovariectomy to construct a model of osteoporosis,and divided into model group,Fisetin 10 mg/kg group,Fisetin 20 mg/kg group,Fisetin 40 mg/kg group and positive control group,with 10 rats in each group.Fisetin group was given different concentrations of Fisetin intervention,and the positive control group was given estradiol(E2)for 8 consecutive weeks.Another 10 rats were selected as sham operation group.Serum alkaline phos-phatase(ALP),osteocalcin(OCN),E2,carboxyl C-terminal cross-linked peptide of type Ⅰ collagen(CTX-Ⅰ),bone γ-carboxyl glutamate protein(BGP)and bone mineral density(BMD)were detected in each group.Bone morphology was ob-served by HE staining.Bone indexes[bone volume fraction(BV/TV),trabecular thickness(Tb.Th),trabecular separation(Tb.Sp),trabecular number(Tb.N)]were detected by micro-CT,and bone biomechanical parameters(femoral neck load-ing force,strength of tibial axis,lumbar compression force)were detected by Micro-CT.The expression levels of Runt-associ-ated transcription factor 2(Runx2),low-density lipoprotein receptor-associated protein-5(Lrp-5),β-catenin and osteoblast-specific transcription factor Osterix(Osx)were detected by Western blot.Results Compared with sham operation group,ALP,OCN,Tb.Sp and CTX-Ⅰ in model group were significantly increased.The levels of E2,BMD,BV/TV,Tb.Th,Tb.N,BGP,femoral neck loading force,strength of tibial axis,lumbar compression force,Runx2,Lrp-5,β-catenin and Osx were significantly decreased(P＜0.05).HE staining showed loose arrangement of trabeculae,destruction of reticular struc-ture,reduction of trabecular number and increase of fracture points.Compared with model group,ALP,OCN,Tb.Sp and CTX-Ⅰin Fisetin 40 mg/kg group and positive control group were significantly decreased.The levels of E2,BMD,BV/TV,Tb.Th,Tb.N,BGP,femoral neck loading force,strength of tibial axis,lumbar compression force,Runx2,Lrp-5,β-catenin and Osx were significantly increased(P＜0.05).HE staining showed that the trabecular arrangement and reticular structure of the femoral bone were obviously restored,and there were higher bone connections.Conclusion Fisetin can improve bone stress in osteoporosis rats after ovariectomy,and its mechanism of action is related to promoting Wnt/β-catenin pathways and reducing rate of high bone turnover.
Keywords：OsteoporosispostmenopausalFisetinOsteocalcinBone mineral densityBone volume fractionFem-oral neck loading forceOsteoblast-specific transcription factorRatsSprague-Dawley
Publication Date：2023-07-22
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 38-43 )