Effects of miR-181a-5p on Cell Proliferation and Apoptosis of T lymphocytic Leukemia Cell Line Jurkat
QIU Ling
FAN Fang-yi
DENG Rui
HE Guang-cui
LIU Yi-lan
MIU Xiao-juan
SU Yi
Abstract：Objective To investigate the effects of miR-181 a-5 p on proliferation and apoptosis of T lymphocytic leukemia (TLL) cell line Jurkat. Methods Jurkat cells were transfected with different combinations of miR-181 a 5 p mimic and pc-B-cell lymphoma 2 (BCL-2) and divided into Jurkat group, mimic-scramble group, miR-182 a mimic group, pc-BCL-2 group and mimic+pc-BCL-2 group. The expression of miR-181 a-5 p and mRNA levels of pc-BCL-2 were measured by RT-PCR, and protein level of BCL-2 was measured by Western Blot. In addition, CCK8 and flow cytometry were performed for cell proliferation and apoptosis. Results Compared with mimic-scramble group, the level of miR-181 a-5 p was up-regulated, while the level of BCL-2 mRNA was down-regulated in miR-181 a mimic group, and the difference is significant (P＜0.01). Compared with Jurkat group, the protein level of BCL-2 and proliferation folds were decreased markedly, while cell apoptosis rate was increased notably in miR-181 a mimic group. In addition, the expression level of BCL-2 protein and proliferation folds were increased, whereas cell apoptosis rate was decreased significantly in pc-BCL-2 group, and there were significant differences (P＜0.01). Compared with miR-181 a mimic group, the protein level of BCL-2 and proliferation folds were increased, while cell apoptosis was reduced in mimic+pc-BCL-2 group, suggesting significant differences (P＜0.01). Conclusion miR-181 a-5 p inhibits cell proliferation and induces apoptosis of TLL cell line Jurkat by targeting inhibition of BCL-2 expression.
Keywords：LeukemiaT-CellCell proliferationApoptosis
Publication Date：2019-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：6( 40-45 )