Effects of Isoliquiritigenin on Learning and Memory Ability and on Oxides in Hippocampal Tissues of Rats after Operation

WU Yan-qiong; XU Xian-cheng; WEI Hui-xia; LI Qing; ZHANG Jian-feng

doi:10.3969/j.issn.1002-3429.2018.07.007

Abstract：Objective To investigate the effect of isoliquiritigenin on learning and memory ability as well as on oxides of hippocampal tissues of rats after operation. Methods Forty male Sprague-Dawley rats were randomly divided into 4 groups: control group(group C, n＝10), operation group(group P,n＝10), low-dose group(group T1, n＝10) and high-dose group(group T2, n＝10). Group P received intraperitoneal injection of 75 mg/kg ket-amine and 5 mg/kg midazolam for anesthesia,and then exploratory laparotomy was performed. Group T1 and T2 were given 10 ml/kg and 20 ml/kg isoliquiritigenin respectively for gastric perfusion, followed by anesthesia after 60 min. Anesthesia and operation were performed as described above in group P. Group C was injected intraperitoneally with the normal saline of the same volume as group P. Cognitive function including learning and memory ability was as-sessed using Morris water maze test 3 h after rats in group P, group T1 and group T2 regained consciousness and 3 h after group C received intraperitoneal injection of 0. 9% sodium chloride solution. Rats were then sacrificed to collect bilateral hippocampal tissues, and the expressions of malondialdehyde( MDA) and superoxide dismutase ( SOD) and glutathione ( GSH) in hippocampus were measured. Results Compared with group C, the latency time was signifi-cantly prolonged and the duration of the target quadrant was significantly shortened in group P. In addition, the ex-pression of MDA was increased significantly, whereas SOD and GSH were decreased ( P<0. 01) . Compared with group P,the latency time was significantly shortened and the duration of the target quadrant was significantly pro-longed in group T1 and T2. Moreover, there was remarkably decreased expression of MDA and notably increased SOD and GSH ( P<0. 01) . Compared with group T2, the latency time was significantly prolonged and the duration of the target quadrant was significantly shortened in group T1. Additionally, the expression of MDA was increased significantly, whereas SOD and GSH were decreased (P<0. 05 or P<0. 01). Conclusion Isoliquiritigenin can ef-fectively improve the ability of learning and memory of rats after operation, the mechanism of which may be related to the inhibition of anti-lipid peroxidation.