Effects of Different Concentrations of Oxycodone on the Biological Behavior of Colorectal Cancer Cells

HE Ya-ni; BI Jian-hong; LI Qing-feng; GAO Xiao-xia; XIE Yuan-yuan

doi:10.3969/j.issn.1002-3429.2018.07.006

Abstract：Objective To investigate the effect of oxycodone on the biological behavior of colorectal cancer ( CRC) cells. Methods SW620 CRC cells were inoculated and incubated for 24 h, followed by treatment with oxy-codone at 0, 10, 20 and 40 μg/ml for 24 h, and then incubation with CCK-8 kit. The microplate reader was used to measure optical density at a wavelength of 490 nm in different concentration groups, to compare the difference in in-hibition rate of cell proliferation. The effects of oxycodone at different concentrations on apoptosis and migration of SW620 cells were detected by flow cytometry FITC-Annexin V/PI and the scratch test respectively. In addition, the impact of different concentrations of oxycodone on the VEGF expression of SW620 cells was detected by ELISA. Re-sults As compared with the control group ( non-oxycodone treatment group) , oxycodone inhibited the proliferation of apoptosis of SW620 cell in different concentrations of oxycodone treatment groups. There were significant differ-ences in apoptosis of SW620 cells in 20 and 40 μg/ml groups, as compared with that in control group ( t＝12. 64, P＝0. 019; t ＝13. 94, P ＝0. 016 ) . The migration distance of SW620 cells was significantly shorter in 20 and 40 μg/ml groups than in control group(t＝11. 29,P＝0. 036;t＝12. 05,P＝0. 009). The expression levels of VEGF in SW620 cells were significantly lower in 20 and 40 μg/ml groups than in control group(t＝12. 62,P＝0. 031; t＝13. 61,P＝0. 013). Conclusion Oxycodone can suppress the proliferation and migration of CRC cells in a dose-de-pendent manner, inhibit VEGF production and secretion, and induce apoptosis of cells.