Studies on the Regulation Mechanism of Smac Polypeptide on Drug Resistance in Lung Adenocarcinoma
CHEN Kang
ZHAI Ai-dong
Nuerlan·ahan
Abstract：Objective To study the effect and mechanism of Smac polypeptide on drug resistance of lungadenocarcinoma cells. Methods Lung adenocarcinoma cell line A549 was cultured and Taxol-resistant A549/Taxol cells were induced. The cells were divided into Smac peptide treatment group ( observation group) and Smac reverse peptide treatment group ( control group) . The proliferative activity of the cell, and expressions of intracellular apop-totic gene and resistance gene were recorded. In addition, the expression levels of apoptotic genes ( Livin, XIAP, Caspase-3, Caspase-7 and Caspase-9), and those of intracellular drug-resistance genes (mRNA of MDR, LRP, To-po-Ⅱ and GST-π) were measured. Results There were significant differences in cell proliferation activity at 12, 18,and 24 hours after treatment in observation group and control group Twenty-four hours after treatment, mRNA ex-pressions of Livin, XIAP, MDR, LRP, Topo-Ⅱand GST-πwere significantly lower in observation group than in con-trol group. Nevertheless, mRNA expressions of Caspase-3, Caspase-7 and Caspase-9 were significantly higher than those of the control group. Conclusion Smac peptide can improve the Taxol resistance of lung adenocarcinoma cells by down-regulating the expression of anti-apoptosis protein and drug resistance genes.
Keywords：Lung neoplasmsDrug resistanceneoplasmInhibitor of apoptosis proteins
Publication Date：2018-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 80-83 )