Inhibition Ability of miR-199a-3p in Proliferation and Invasion of SK0V3 Cells in Ovarial Cancer and Its Mechanism
ZHANG Li-juan
ZHANG Xu-li
WANG Juan
WANG Fang
Abstract：Objective To investigate effects of miR-199a-3p in proliferation and invasion of SK0V3 cells in ovarial cancer and its mechanisms. Methods SK0V3 cell line of ovarial cancer were recruited in this study, and SK0V3 cells in log-arithmic growth phase were divided into miR-199a-3p overexpression, miR-199a-3p inhibition group and control groups. The miR-199a-3p overexpression and inhibition rates, and its effects on proliferation and invasion of SK0V3 cells and Met, MMP-2 and CD44 protein expressions were observed. Results Compared with those in control group, in miR-199a-3p overexpression group, miR-199a-3p in SK0V3 cancer cells was over expressed, and numbers of SK0V3 cells, cell clone formation and inva-sion cells, expressions of c-Met, MMP-2 and CD44 of c-Met downstream proteins were significantly decreased; in miR-199a-3p inhibition group, miR-199a-3p expression in SK0V3 cancer cells was decreased, and numbers of SK0V3 cells, cell clone formation and invasion cells,protein expressions of c-Met, MMP-2 and CD44 of c-Met downstream proteins were sig-nificantly increased (P<0. 05). Conclusion Thet miR-199a-3p can affect invasion and transference of SK0V3 cells in ovar-ial cancer by inhibiting c-Met, MMP-2 and CD44 protein expressions, and therefore miR-199a-3p may be used as a potential target in treatment of ovarian cancer.
Keywords：Ovarian cancermiR-199a-3pOncogeneCell proliferationNeoplasm invasiveness
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 90-93 )