Expressions and Clinical Significances of OX40, OX40L, PD-1, PD-L1 and SPD-1 in Peripheral Blood of Patients with Primary Sjogren's Syndrome
ZHENG Ge
YUAN Yi-meng
Abstract：Objective To investigate expressions and clinical significances of OX40, OX40L, PD-1, PD-L1 and SPD-1 in peripheral blood of patients with primary Sjogren's syndrome ( PSS) . Methods A total of 50 patients with PSS ( ob-servation group) admitted during March 2012 and March 2015 were divided into active period subgroup (n=30) and inactivi-ty period subgroup (n=20) according to disease activity conditions, and single system subgroup (n=29) and multisystemic subgroup (n=21) according to active stages. Other 50 healthy people taking medical examinations at the same period were selected as control group. OX40, OX40L, PD-1, PD-L1 and SPD-1 expressions in peripheral venous blood were detected by immunofluorescence labeling and flow cytometry methods in two groups. OX40, OX40L, PD-1, PD-L1 and SPD-1 expressions were compared in groups and subgroups, and changes of the above indexes before and after treatment were recorded in observa-tion group. Results In observation group, CD4 +OX40 +, CD14 +OX40L+, CD19 +OX40L+, CD4 +PD-1 + and CD4 +PD-1L+ and SPD-1 expressions were significantly higher, while CD14 +PD-L1 + was significantly lower than those in control group (P<0. 01). CD4 +OX40 +, CD14 +OX40L+, CD19 +OX40L+, CD4 +PD-1 + and SPD-1 expressions in active period sub-group were significantly higher than those in inactivity period subgroup ( P <0. 05 or P <0. 01 ); CD4 +OX40 +, CD14 +OX40L+, CD19 +OX40L+ and SPD-1 expressions in single system subgroup were significantly lower than those in multisys-temic subgroup ( P<0. 05 or P<0. 01 ) . In 10 preliminary diagnosis patients in observation group, CD4 +OX40 +, CD14 +OX40L+, CD19 +OX40L+ and CD4 +PD-1 + expressions before treatment were significantly higher than those after treatment ( P<0. 05);there were no significant differences in CD8 +PD-1 +, CD14 +PD-L1 + and SPD-1 expression between before and after treatment (P>0. 05). Conclusion Abnormal expressions of OX40, OX40L, PD-1, PD-L1 and SPD-1 in peripheral venous blood of PSS patients relate to disease activity and tissue damage, and the expressions can be used as detection indexes for learning disease activity conditions and severe degree.
Keywords：Sjogren's syndromePathogenetic conditionOX40OX40LPD-1PD-L1
Publication Date：2017-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 83-87 )