Effects of Diammonium Glycyrrhizinate on the Expression of HMGB1 in Alveolar Epithelial Cell with Paraquat

FANG Chen; ZHANG Jian-feng; YU Li; XIANG Li

doi:10.3969/j.issn.1002-3429.2017.04.035

Abstract：Objective To investigate the effects of Diammonium Glycyrrhizinate (DG) on the expression of HMGB1 in rat alveolar epithelial cell Ⅱ(AECⅡ)with paraquat (PQ). Methods The AECⅡ was cultured in RPMI-1640 media containing 10% FBS, 100 U/ml penicillin and 100 μg/L streptomycin at 37°C in 5% CO2.The cells were divided into three groups: The NS group was induced 24 h without any drugs, the PQ group was induced 24 h by 1000 μmol/L PQ ,and the DG group was induced 2 h by 0.6 mg/ml DG, then, was induced 24 h by 0.6 mg/ml DG+1000 μmol/L PQ.MTT assay was used to measure the proliferation effect of DG on AEC Ⅱ with PQ.The ELISA assay was applied to measuring the levels of HMGB1, TLR-4,MyD88,NF-κB, TNF-α in the three groups.The gene expressions of HMGB1,TLR-4, MyD88, NF-κB P65 mRNA in the three groups were detected by R T-PCR.Results The highest survival rate of AECⅡ induced 24 h by 1000 μmol/L PQ was in the group of 0.6 mg/ml DG, the lowest survival rate of AECⅡ induced 24 h by 1000 μmol/L PQ was in the group of 0 mg/ml DG.The levels of HMGB1, TLR4, MyD88, NF-κB P65, TNF-α in PQ and DG group were higher than those in NS group.While, the levels of HMGB1, TLR-4, MyD88, NF-κB P65, TNF-α in DG group were lower than those in PQ group (P<0.01).The expression of HMGB1, TLR-4, MyD88, NF-κB P65, TNF-α mRNA in PQ and DG group were higher than those in NS group.The expression of HMGB1, TLR4,Myd88,NF-κB P65,TNF-αmRNA in DG group were lower than those in PQ group(P<0.01). Conclusion DG can attenuate the injury in rat alveolar epithelial cells caused by paraquat poisoning.And it can decrease the expressions of HMGB1, TLR-4, MyD88, NF-κB and TNF-α.