Negative Pressure Wound Therapy Associated with Up-regulation of VEGF in Granulation Tissues of Human Diabet-ic Foot Wounds
SUN Lei-lei
YANG Shao-ling
LI Xiao-ling
ZHU Lv-yun
Abstract：Objective To explore the expression of the vascular endothelial growth factor (VEGF) of diabetic foot wound granulation tissues in negative pressure wound therapy ( NPWT) . Methods 40 patients with diabetic foot ulcers dur-ing January 2010 and December 2014 fitting in the inclusion criteria were enrolled in our study and divided into control group (n=20) treated with conventional gauze therapy and the experimental group (n=20) treated with NPWT, according to com-puter randomized algorithm. Granulation tissue biopsies were taken before (day 0) and after (day 12) treatment respectively. HE staining was applied to observe the growth of the granulation tissue and micro vessel density ( MVD) . The expression of VEGF was evaluated with immunohistochemical staining and Western Blot. Independent sample test was used for statistical analysis. Results Compared with the control group, there were more fresh granulation tissues in the NPWT group, with for-mation of more neovascularizations, which was statistically proved by a significant increase in MVD (t=19. 76,P<0. 01;t=4. 29,P<0. 01). Immunohistochemical analysis revealed that there was a more significant up-regulation in the NPWT group compared with that of the control group (t=13. 34, P<0. 01), which was further confirmed by Western blot analysis (t=13. 07, P<0. 01). Conclusion Compared with conventional gauze therapy, NPWT can promote the up-regulation of VEGF expression of granulation tissues, and subsequently help wound healing.
Keywords：Negative-pressure wound therapyDiabetic footVascular endothelial growth factor A
Publication Date：2016-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 71-75 )