An Experimental Study on the Treatment of Bronchial Asthma from the External and External Organs of the Lungs

WANG Bao-kai; YANG Chao; ZHAO Ji-ping

doi:10.3969/j.issn.1002-3429.2015.08.035

Abstract：Objective To observe expression effect of acupuncture treatment on the surface protein ( SP)-A mRNA expression of brain, liver and kidney tissues in asthmatic rats, and to explore the approach of bronchial asthma from the exter-nal and external organs of the lungs. Methods 40 SD rats were randomly divided into blank group, model group, lung acu-puncture group, intestinal acupuncture group, lung and intestinal acupuncture group. Methods of observation of model group, lung acupuncture group, intestinal acupuncture group, lung and intestinal acupuncture group were proceeded through the in-jection of ovalbumin sensitization in the abdomen cavity and after 2 weeks, intravenous injection of the ovalbumin was carried out to induce asthma. Method of observation of blank group was proceeded through injection 0. 9% sodium chloride injection at the same time points of other groups. Lung acupuncture group selected the following acupoints: Chize ( LUS ) , Kongzui (LU6), Lieque (LU7), Feishu (BL13). In large intestine acupuncture group, the following acupoints were selected:Quchi (LIll), Hegu (LI4), Tianshu (ST25), Shangjuxu (ST37) were selected. In lung and large intestine acupuncture groups, the following acupoints were selected, Kongzui (LU6), Lieque (LU7), Quchi (LIll), Tianshu (ST25). The expression of SP-A mRNA in brain, liver and kidney tissues was observed after the rats were killed. Results The expression of mRNA SP-A was found in brain, liver and kidney in each group. The expression of mRNA SP-A in the brain tissues of the blank group, the acupuncture group, the acupuncture group and the acupuncture group were significantly higher than that of the model group (P<0. 01). The levels of SP-A mRNA in brain tissues of rats with acupuncture group were significantly higher than that in the acupuncture group (P<0. 05). There was no significant difference in SP-A mRNA expression in liver and kidney tissues between the three groups (P>0. 05). Conclusion SP-A mRNA abnormal expression in brain tissues of rats in asthma and acupuncture lung and colorectal system acupoints may have an impact on the expression of SP-A mRNA in brain tissues. The effect of acupuncture on the expression of mRNA SP-A in the liver and kidney tissues of asthmatic rats is not obvious. Acu-puncture in the treatment of asthma, regulating the law of regulation spirit can be used in treatment of any diseases, but may not apply to treatment of new onset of asthma from the liver and kidney treatment theory.