Effects of Promoting Healing and Scar Inhibition of Epidermal Growth Factor Film on Healing of Skin Incision Wounds

ZHAO Li; LIANG Xiao-long; ZHANG Yu-jie; LI Chuang; WU You-yu

doi:10.3969/j.issn.1002-3429.2015.08.034

Abstract：Objective To study the effect of promoting healing and scar inhibition of epidermal growth factor ( EGF) film on the dermal wound healing of rats. Methods 36 SD rats were randomly divided into treatment group and control group ( n=18 each group) . Under general anesthesia, two dermal incisions on both sides of the spine were as deep as the muscle layers. The incisions of two groups were applied with black film and epidermal growth factor ( EGF) film respectively. All films were changed daily to avoid infection. For each group three rats were killed on 3, 5, 7, 9, 14, 21 day after surgery and samples were obtained. The dermal incision was observed by visual and the histological study. In brief, incision changes were investigated by masson's trichrome staining and the expression of EGF in skin was detected with Streptavidin Peroxidase ( SP) immunohistochemical methods (not performed 14, 21 d after injury ). The content of PCⅢ was also determined. Results The healing velocity of epidermal and dermis wounds were quicker in treatment group than that in control group. In terms of masson's trichrome staining:the new collagen fibers displayed smaller quantity, slimmer and looser arrangement in treatment group compared with that of control group. The epidermal of control group showed positive EGF staining on the 5th and 7th day, negative at the 3rd and 9th day. However, in treatment group, the upper part of the epidermal showed stronger positive EGF staining on the 3rd, 5th, 7th, 9th day. The content of PCⅢ in EGF film group was higher than that of the control group on all the days, except on the 21st day (P<0. 05). Conclusion The film made from EGF can enhance the content of EGF in wounds, improve wound healing process and repress scar. formation.