A Study of the Protective Effects and Mechanisms of Ramipril on Kidney in Diabetic Rats

WANG Jing-hua; YANG Xin-jun; LIU Yu; CHEN Yun-shuang; LIN Jing; YANG Jie; LIU Ying

doi:10.3969/j.issn.1002-3429.2015.01.041

Abstract：Objective To observe the changes of renal pathology and the protein expression of Megsin, PAI-1 and IGF-1 with the ramipril intervention, in order to study the protective mechanisms of ramipril. Methods 48 healthy male SD rats were randomly divided into three groups:normal control group (Group A, n=12);diabetic rat models were established with peritoneal injection of streptozotocin ( blood glucose≥16. 7 mmol/L) and the successful 24 models were divided into dia-betic group (Group B, n=12) and ramipril intervention group [1 mg/(kg·d), Group C, n=12]. 12 weeks later, the blood, urine and kidney samples were collected for further analysis; the weight, 24 h urine, blood glucose and kidney/body weight ratio, the amount of 24 h urinary protein and serum creatinine clearance rate were compared. The histopathological changes of renal tissues were also observed. And the protein expression of Megsin, PAI-1 and IGF-1 were respectively meas-ured by immunohistochemistry and western blot. Results Compared with that of Group A, the body weight loss was greater, the 24 h urine, blood glucose and kidney/body weight ratio and serum creatinine clearance rate increased more in Group B and C with statistical significance (P<0. 05). The 24 h urine and serum creatinine clearance rate were obviously decreased in Group C (P<0. 05). There was no significant renal pathological change in Group A, while there was in Group B. Mean-while, in Group B, the mesangial area became wider, PAS stain positive materials and the expression of Megsin, PAI-1 and IGF-1 increased more significantly than that in Group A (P<0. 05). Conclusion Ramipril can alleviate pathological changes of diabetic rats, and lower the expression of Megsin, PAI-1, IGF-1 in renal tissues, and with a protective effect.