A Sensitivity Study of HDAC1 RNA to Enhance Chemosensitivity in Human Cervical Carcinoma Cell Line HeLa

KONG Xiang-ling; WU Wei-guang; SHE Ye; GE Hong-yu

doi:10.3969/j.issn.1002-3429.2015.01.40

Abstract：Objective To observe histone deacetylase 1 (HDAC1) expression inhibited by siRNA in vitro on chemo-sensitivity to cisplatin in human cervical carcinoma cell line HeLa, and to investigate the possible mechanisms. Methods The siRNA targeting HDAC1 was generated in vitro, and transfected into human cervical carcinoma HeLa cell by lipo-fectamine. The HeLa cells were divided into normal control group ( no intervention) , negative control group ( transfected with negative control siRNA) , and siRNA group ( transfected with HDAC1 siRNA) . Western blot was used to detect the HDAC1 protein and acetyl level of histone H4 (Ac-H4), and real time PCR was used to detect mRNA of HDAC1 and NF-κB. The cells were treated under different density DDP. The cell viability was detected by methyl thiazolyl tetrazolium ( MTT) assay and 50% inhibitive concentration ( IC50 ) and sensitivity of DDP were examined, and the apoptosis rate was measured by flow cytometry. Results Compared with those in control groups and negative control group, the expressions of HDAC1 gene pro-tein and mRNA and NF-κB mRNA decreased (P<0. 05 or P<0. 01), and the Ac-H4 increased in siRNA group (P <0. 05). After being treated with different concentrations of DDP, the IC50 to DDP and apoptotic rates in siRNA group were higher than those in other groups (P<0. 05 or P≤0. 01). Conclusion The siRNA targeting HDAC1 can enhance chemo-sensitivity to DDP in cervical cancer HeLa cells. Suppressing the expression of HDAC1 and increasing acetyl level of histone to down-regulate NF-κB gene expression may be its mechanisms.