Inhibitory Effects of Kiwi Essence on the Growth of Lung Adencarcinoma in Mice

DENG Xiang; HE Jian-bin; ZHANG Ping

doi:10.3969/j.issn.1002-3429.2014.12.041

Abstract：Objective To observe the inhibitory effect of Kiwi essence on xenograft growth of lung adenocarcinoma in mice. Methods Forty C57BL/6J mice bearing Lewis cells were randomized into five groups: control group (0. 9% sodium chloride) , Kiwi essence group ( Kiwi essence 180 mg/kg) , chemotherapy group ( cisplatin and gemcitabine) , low-dose Kiwi essence combined chemotherapy group ( Kiwi essence 60 mg/kg combined cisplatin and gemcitabine) , high-dose Kiwi essence combined chemotherapy group (180 mg/kg Kiwi essence combined cisplatin and gemcitabine). 4 days after being inoculated with adenocarcinoma cells, drug intervention was given in five groups of mice. Mice were sacrificed at day 20 after tumor inoc-ulation. The subcutaneous tumors were stripped for histological examination. The tumor inhibitory rate was measured. The ex-pressions of vascular endothelial growth factor ( VEGF) , Bcl-2 and Survivin were determined by immunohistochemistry method with image analyzing system. Results There were significantly lower values in subcutaneous tumor volume and weight in drug-treated groups. The inhibitory rate was 14. 95%, 41. 77%, 44. 21% and 59. 68% in Kiwi essence group, chemotherapy group, low-dose Kiwi essence combined chemotherapy group, high-dose Kiwi essence combined chemotherapy group respec-tively (P<0. 05, P<0. 01). VEGF, Survivin, Bcl-2 in each group were lower than in the control group (P<0. 05, P<0. 01). Conclusion Kiwi essence can produce a synergetic inhibitory activity against tumor growth of lung adenocarcinoma and enhance GP therapeutic regimen ( cisplatin plus gemcitabine) to inhibit tumor growth of lung adenocarcinoma, which may be associated with the effect of suppressing the expression of angiogenesis, Survivin and Bcl-2.