Effect of Skp2 shRNA on the Chemosensitivity of Laryngeal Squamous Carcinoma Cells
LU Xiu-Ying
LI Xiao-Ming
Abstract：Objective To investigate the effect of silencing of S-phase kinase associated protein 2 (Skp2) by small hairpin RNA ( shRNA) in combination with chemotherapy on proliferation and apoptosis in laryngeal carcinoma cell line Hep-2 cells. Methods Laryngeal cancer cells were divided into pGPU6 group, Skp2 shRNA group, Skp2 shRNA with chemotherapy group, pGPU6 with chemotherapy group and chemotherapy group. After transfected with Skp2 shRNA, Hep-2 cells were cultured and received cisplatin 6 h after that. Forty-eight hours after cultured, the cell proliferation was assayed by MTT, and apoptosis rate and the expression of Skp2 and p27 protein were analyzed by flow cytometry. Results The expression of Skp2 protein in Skp2 shRNA group was significantly lower than that in pGPU6 group (P<0. 01). The expression of p27 protein in Skp2 shRNA group was higher than that in pGPU6 group (P<0. 05). The growth inhibition rate in Skp2 shRNA group with cisplatin at 0 mg/L, 1 mg/L, 2 mg/L and 3 mg/L were higher than those in pGPU6 group with cisplatin at 0 mg/L, 1 mg/L, 2 mg/L and 3 mg/L, respectively (P<0. 01). The apoptosis rate in Skp2 shRNA with cisplatin group (2 mg/L) was strikingly higher than that in pGPU6 with cisplatin group (2 mg/L)(P<0. 01). Conclusion Skp2 shRNA could inhibit the expression of Skp2 gene selectively and combining with chemotherapy might provide a new therapeutic approach to treatment of laryngeal carcinomas.
Keywords：Laryngeal neoplasmsNeoplasmssquamous cellS-phase kinase-associated proteinsDrug therapy
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 36-39 )