Sodium Glycididazole Enhancing Radiosensitivity of Laryngeal Cancer Cells through Down-Regulation of the ATM Expression
ZENG Yue-can
WU Rong
CHI Feng
XING Rui
WANG Si-liang
CAO Shuo
NIU Nan
TANG Mei-yue
XIAO Yu-ping
Abstract：Objective To evaluate the expression of ataxia-telangiectasia mutated (ATM) gene in laryngeal cancer cells after the intervention of combined sodium glycididazole and radiation. Methods Laryngeal cancer Hep-2 cells were di-vided into the normal group, the irradiation group (5 Gy of X-radiation) and the observation group (irradiation plus sodium glycididazole group) . In observation group, laryngeal cancer cells received 5 Gy of X-radiation at 4 h after the intervention of 100 μl of sodium glycididazole (concentration of 0. 8 mmol/L). The ATM mRNA and protein expression of laryngeal cancer cells were detected by RT-PCR and Western blot hybridization, respectively. Results The ATM mRNA expression levels in normal group, irradiation group and observation group were 0. 727±0. 024, 0. 955±0. 046 and 0. 272±0. 038, respectively. The ATM mRNA and protein expression of laryngeal cancer Hep-2 cells declined after the intervention of sodium glycididazole. The differences of ATM mRNA and protein expression levels among the three groups were significant (P<0. 05). Conclusion Sodium glycididazole could enhance radiosensitivity of laryngeal cancer cells through down-regulation of the ATM expres-sion.
Keywords：Laryngeal neoplasmsSodium glycididazoleRadiotherapyRadiation-sensitizing agents
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 29-32 )