Effect of Di-2-ethylhexyl Phthalate on Tight Junction and Related Gene Expressions of Sertoli Cells in Vitro in Rats
KONG Xiang-ling
SHE Ye
GE Hong-yu
WU Wei-guang
Abstract：Objective To explore the effect of di-2-ethylhexyl phthalate ( DEHP) on tight junction and related gene ex-pression of sertoli cells in vitro in rats. Methods The rats' sertoli cells were separated in vitro and primarily cultured, and dual-chamber models for sertoli primary culture were established, and then sertoli cells were treated with different concentrations of DEHP (10, 50 and 100 nmol/L) for 24 h. The transepithelia electrical resistance (TER) assay was used to detect TER value;fluorescent quantitation polymerase chain reaction (FQ-PCR) was used to detect mRNA of Occludin, Zonula occluden-1 (ZO-1) and Claudin 11 gene expressions, and the Western blot method was used to detect the protein expressions of Occludin, ZO-1 and Claudin 11. Results Compared with those in control group, the TER values decreased in different concentrations DEHP groups. Meanwhile, the Occludin, ZO-1 and Claudin 11 gene of mRNA expressions decreased with increasing DEHP concentra-tion (P<0. 05), and the Occludin, ZO-1 and Claudin 11 protein expressions also decreased with increasing DEHP concentra-tion (P<0. 05). Conclusion DEHP can downregulate Occludin, ZO-1 and Claudin 11 gene expressions to affect tight junction of sertoli cells in rats, which may affect sertoli cell function.
Keywords：Diethylhexyl phthalateTestisSertoli cellTight junctionGene expression
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 108-111 )