Protective Effect of Recombinant Human Basic Fibroblast Growth Factor on Retinal Ischemia-reperfusion Injury in Rabbits
SHAO Hong-chao
GE Yan-ran
LI Li-yan
WANG Lin-hong
MA Jian-ying
CAO Feng-ying
TIAN Hua
Abstract：Objective To explore the protective effect of recombinant human basic fibroblast growth factor (rh-bFGF) on retinal ischemia-reperfusion injury (RIRI) in rabbits. Methods The 66 healthy purebred rabbits were randomly divided into sham-operation group (n=6), ischemia-reperfusion model group (n=30) and rh-bFGF treatment group (n=30). The RIRI models were established by elevating intraocular pressure, the ischemia-reperfusion model group was injected with 0. 9% sodium chloride injec-tion;rh-bFGF drug therapy was performed in rh-bFGF treatment group;the sham-operation group only received the 5th scalp heedle from ear piercing the anterior chamber angle in front of the sclera edge, and then the needle was slowly removed. Retina histopatho-logical changes were observed by routine hematoxylin and eosin stain 6 h, 12 h, 24 h, 48 h and 72 h after RIRI in ischemia-reperfu-sion model and rh-bFGF treatment groups. Expressions of inhibiting gene Bcl-2 and stimulative gene Bax and changes of Bax/Bcl-2 in retinal tissues were detected with SABC immunohistochemical method. Results No apoptotic cell was found in sham-operation group;apoptotic cells mainly existed in the inner nuclear layer and the ganglion cell layer, and apoptotic cells reached the peak 24 h after reperfusion;the rh-bFGF treatment groups had the similar condition with the ischemia-reperfusion model group in cell in-jury at each observational time. No expressions of Bcl-2 and Bax positive cells were found in normal retina tissues;the expressions were found 6 h after reperfusion, and the expressions reached the peak 24 h after reperfusion, and began to descented 48 h after reperfusion, and the decrease was obvious 72 h after reperfusion. Bcl-2 expression was significantly stronger, while Bax expression was significantly reduced in rh-bFGF treatment group, compared with those in ischemia-reperfusion model group (P<0. 05). Con-clusion Bcl-2 and Bax protein are involved in the RIRI regulation. The retina tissue can be protected with Bcl-2 expression of up-regulation and the Bax expression of down-regulation by rh-bFGF, which can increase the Bcl-2 /Bax ratio.
Keywords：Recombinant human basic fibroblast growth factorRetinaReperfusion injuryBcl-2Bax
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：5( 100-104 )