Value of Tuberculosis Protein Chip Method in Diagnosis of Elderly Patients with Pulmonary Tuberculosis
HE Ying-wu
Abstract：Objective To investigate the value of tuberculosis protein chip method in diagnosis of elderly patients with pulmonary tuberculosis. Methods A total of 93 elderly patients with pulmonary tuberculosis and 77 patients with nonspecific lung infection were recruited in this study, and the antibodies of mycobacterium tuberculosis ( MTB) lipoarabinomannan ( LAM) and genetic engineering custom-crafted 16 kD and 38 kD-IgG were detected by using diagnostic kit for IgG antibody to mycobac-terium tuberculosis and acid-fast staining test of sputum smear, The sensitivity and specificity of the two methods in diagnosis of mycobacterium tuberculosis infection were calculated respectively, and the receiver operating characteristic curves were built re-spectively, and then the diagnostic values were evaluated by comparing the areas under the curves ( AUC) . Results The sensi-tivity and specificity of tuberculosis protein chip method and acid-fast staining test of sputum smear in diagnosis of mycobacterium tuberculosis infection were 68. 82% &33. 33% and 100. 00% &97. 40% respectively;the AUC was 0. 84 and 0. 65 respective-ly, which showed that the diagnostic value of tuberculosis protein chip method was much better when compared with that of acid-fast staining test of sputum smear, and the differences were of statistical significance (P<0. 01). Conclusion The tuberculosis protein chip method in detection of LAM, 16 kD and 38 kD-IgG antibodies of mycobacterium tuberculosis is simple, quick and has significant practical value in diagnosis of elderly patients with pulmonary tuberculosis.
Keywords：Protein chipAntibodyMycobacterium tuberculosisTuberculosispulmonaryDiagnosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 47-49 )