Effect of Induced Pluripotent Stem Cell in Treatment of Diabetic Foot Ulcers in Rats
GUO Jing-Xia
LEI Lin
REN Qiao-Hua
WANG Xin
Abstract：Objective To observe the curative effect of allogeneic induced pluripotent stem cells( IPSC)in treatment of diabetic foot ulcers in SD rats by local transplantation,and the effect on vascular endothelial growth factor( VEGF). Meth-ods A total of 40 male SD rats were randomly divided into IPSC treatment group(n=20)and control group(n=20). Rat models of diabetic foot ulcers were established in the two groups. In the IPSC treatment group,ulcer wounds were multipointly injected with IPSC suspensions into tunica dartos retia,while the control group was injected with the same volume of Knockout DMEM culture solution. The ulcer areas on the 1st d,5th d and 10th d of treatment and VEGF levels in peripheral blood of the two groups were observed and comparedResults The 40 SD rat models were successfully established. There was no significant difference in ulcer area on the 1st d of treatment in the two groups(P﹥0. 05);ulcer areas in IPSC treatment group on the 5th d and 10th d of treatment were significantly smaller compared with those in control group(P﹤0. 05). There was no significant difference in VEGF level of peripheral blood on the 1st d of treatment in the two groups(P﹥0. 05);levels of peripheral blood in IPSC treatment group on the 5th d and 10th d of treatment were significantly higher than those in control group(P﹤ 0. 05). Conclusion IPSC allotransplantation may promote the healing of diabetic foot ulcers and local neovascularization in SD rats.
Keywords：Induced pluripotent stem cellRatDiabetes mellitusUlcerVascular endothelial growth factor
Publication Date：2014-05-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 101-104 )