BaP Effect on the Activities of Cytochrome P450,GSH and PX in Placental Tissues of Rats and the Concentration of BPDE-DNA in Peripheral Blood of Fetal Rats
ZHU Shu-ping
HOU Hai-yan
FU Hong-wei
Abstract：Objective To explore the benzo[a]pyrene(BaP)effect on the activities of cytochrome P450(CYP450) and glutathione peroxidase(GSH-PX)in placental tissues of rats and the concentration of BPDE-DNA[benzo(a)pyrene-7,8-Dihydrodiol-9,10-Epoxide,BPDE]in peripheral blood of fetal rats. Methods Viripotent 120 SD rats were randomly divided into control group(n=30),low concentration BaP group(n=30),middle concentration BaP group(n=30)and high con-centration BaP group(n=30),and female rat and male rats were raised respectively. Rats were lavaged with olive oil in con-trol group,and different concentrations BaP(0. 5,1. 0 and 2. 0 mg/kg)in the other three groups respectively,and males were mated with females after treatment for 15 d. Rats,which were found with semen plug at the second day,were continuous-ly lavaged with the same methods previous for 14 d and killed,and then the activities of CYP450 and GSH-PX in placental tis-sues and the concentration BPDE-DNA in peripheral blood of fetal rats were detected. Results The activity of CYP450 and the concentration of BPDE-DNA in BaP groups were significantly higher,while the GSH-PX activity was significantly lower than those in control group(P﹤0. 05). In BaP groups,the activity of CYP450 and the concentration of BPDE-DNA gradually increased with the increased BaP dose,while the GSH-PX activity gradually decreased with the increased BaP dose,and the differences in the three groups were statistically significant(P ﹤0. 05). The correlation analysis showed that the activity of CYP450 was negatively correlated with the GSH-PX activity,and was positively correlated with BPDE-DNA adduct concentra-tions. Conclusion BaP may enhance the activity of CYP450 and the concentration of BPDE-DNA in peripheral blood of fetal rats,and decrease the GSH-PX activity in placental tissues.
Keywords：Benzo(a)pyreneRatPlacentaCytochrome P450Glutathione peroxidaseDNA adducts
Publication Date：2014-05-02
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：4( 98-101 )