Passive Agglutination Method in Detection of False Negative Mycoplasma Pneumoniae Antibody Induced by Hook Effect
ZHANG Xiang-feng
Abstract：Objective To explore the causes and countermeasures of passive agglutination method in detection of false negative mycoplasma pneumonia( MP)antibody induced by hook effect so as to improve the understanding of hook effect in the clinical inspection. Methods The serum of a male child was detected by times of dilution according to the kit′s re-quirements,and then was observed having stood for 3 hours after plusing the sensitization. Results The reaction holes of 1∶(40-160)dilutions of the child were negative,the reaction holes of 1∶ (320-5120)dilution were positive,and the reaction holes of 1∶ (10240-20480)dilution were negative. The double-checked results were the same. Conclusion The passive ag-glutination method in detection of false negative MP antibody induced by hook effect is rare and may easily be missed diagnosis or misdiagnosed. Lab staff should carry out comprehensive analysis combined with the case history and other testing results if the results are suspicious.
Keywords：Mycoplasma pneumoniaeHook effectAgglutination testFalse negative reactionMisdiagnosis
Publication Date：2014-01-01
Online Publishing Date：2025-08-15(First online date of this platform, not the publication date of the document)
Pages：3( 64-66 )