Effect of ethanol extract of propolis on m6A methylation in colon cancer HCT116 cells
JI Hongxiu
LIU Zhengxin
XUAN Hongzhuan
WANG Meng
Abstract：This study investigates the inhibitory effects and underlying mechanisms of ethanol extract of Chinese propolis(EECP)on the proliferation of human colorectal cancer cells(HCT116)through modula-tion of N6-methyladenosine(m6A)RNA methylation.HCT116 cells were treated with varying concentra-tions of EECP(25,50,and 100 μg/mL).Cell proliferation was assessed using the CCK-8 assay,while the global m6A modification levels of total RNA were measured by dot blot analysis.The mRNA and protein expression levels of Methyltransferase-like 3(METTL3),Methyltransferase-like 14(METTL14),Wilms Tumour 1-Associating Protein(WTAP)and Fat Mass and Obesity-associated Protein(FTO)were evalua-ted using real-time quantitative PCR(RT-qPCR)and Western blot,respectively.The results demonstra-ted that increasing concentrations of EECP significantly reduced the total RNA m6A levels in HCT116 cells(p＜0.05).Additionally,EECP treatment led to decreased expression levels of FTO,WTAP and METTL3,while METTL14 expression was significantly upregulated(p＜0.05).In conclusion,EECP induces alterations in m6A methylation and related enzyme expression in HCT116 cells,suggesting that EECP may exert anti-colorectal cancer effects by regulating m6A methylation.
Keywords：colon cancerpropolism6AHCT116
Publication Date：2025-12-31
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：9( 988-996 )