The impact mechanism of iron on the anaerobic degradation of 3,5,6-trichloro-2-pyridinol by Pseudomonas aeruginosa
LÜ Xueru
GAO XiJun
DING Xiaoke
WANG Shenghui
Abstract：To explore the impact mechanism of NanoFe3O4 and Fe(Ⅱ)ions on the anaerobic degradation of 3,5,6-trichloropyridinol(TCP)by Pseudomonas aeruginosa D-2.An anaerobic co-culture system was set up with three treatments:no iron control group(A,0 g/L),NanoFe3O4 treatment(B,2.5 g/L)and Fe(Ⅱ)treatment(C,2.5 g/L).The degradation performance of TCP in different groups was detected using HPLC.The differential gene expression screening and metabolic pathway enrichment analysis of the three treatments were conducted via transcriptome sequencing.Within 72 hours,the degradation rates of 50 mg/L TCP for the three treatments were 54.08％(A),40.20％(B),and 60.24％(C),respectively.NanoFe3O4 inhibited TCP degradation,but Fe(Ⅱ)ions significantly accelerated TCP degradation,increas-ing its degradation rate by 14.16％.Transcriptomic analysis showed that NanoFe3O4 enriched some path-ways related to DNA damage stimuli,cell stress responses and DNA damage repair,and upregulated genes associated with DNA damage stress response and DNA repair.While,Fe(Ⅱ)significantly enriched some pathways related to energy metabolism such as ribosomes,TCA cycle,and oxidative phosphoryla-tion,and upregulated iron-dependent enzyme.NanoFe3O4 triggered the emergency damage repair system of strain D-2 and initiated the protective mechanism,leading to a 9.78％decrease in the degradation rate of TCP.Fe(Ⅱ)ions further activated energy metabolism by up-regulating the expression of key enzymes,thereby resulting in a 14.1％increase in the degradation rate of TCP.
Keywords：NanoFe3O4Fe(Ⅱ)356-trichloropyridinePseudomonas aeruginosaanaerobicdegrada-tiontranscriptomicsmechanism
Publication Date：2025-10-25
Online Publishing Date：2026-08-28(First online date of this platform, not the publication date of the document)
Pages：8( 773-780 )